EHMT2 / G9A Recombinant Rabbit Monoclonal Antibody [JE50-84]
cat.: ET7110-08
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE50-84 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 132 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human G9A aa 1,060-1,210 / 1,210. |
| Positive control: | Human colon carcinoma tissue, human breast carcinoma tissue, human stomach carcinoma tissue. |
| Subcellular location: | Nucleus, chromosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50-1:400 |
| Uniprot #: | SwissProt: Q96KQ7 Human |
| Alternative names: | Ankyrin repeat containing protein Bat 8 Bat8 C6orf30 DKFZp686H08213 EHMT 2 Ehmt2 EHMT2_HUMAN Euchromatic histone lysine methyltransferase 2 Euchromatic histone lysine N methyltransferase 2 Euchromatic histone-lysine N-methyltransferase 2 FLJ35547 G 9a G9 a G9a protein G9A G9A histone methyltransferase GAT8 H3 K9 HMTase 3 H3-K9-HMTase 3 Histone H3 K9 methyltransferase 3 Histone H3 K9 methyltransferase3 Histone H3-K9 methyltransferase 3 Histone lysine N methyltransferase Histone lysine N methyltransferase EHMT2 Histone lysine N methyltransferase, H3 lysine 9 specific 3 Histone lysine N methyltransferase, H3 lysine 9 specific3 Histone-lysine N-methyltransferase EHMT2 HLA B associated transcript 8 HLA-B-associated transcript 8 KMT 1C KMT1 C Lysine N methyltransferase 1C Lysine N-methyltransferase 1C NG 36 NG36 Protein G9a |
Images
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Fig1:
Western blot analysis of EHMT2 / G9A on different lysates with Rabbit anti-EHMT2 / G9A antibody (ET7110-08) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-EHMT2 / G9A KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 132 kDa Observed band size: 160, 170 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-08) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-EHMT2 / G9A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-EHMT2 / G9A antibody (ET7110-08) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-EHMT2 / G9A antibody (ET7110-08) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.