Phospho-Met (Y1349) Recombinant Rabbit Monoclonal Antibody [JE51-68]
cat.: ET7110-14
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE51-68
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 156 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr1,349 of human Met.
Positive control: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, human colon carcinoma tissue, human small intestine tissue, human rectum tissue.
Subcellular location: Membrane, secreted.
Recommended Dilutions:
  WB
  IHC-P
1:1,000
1:50-1:200
Uniprot #: SwissProt: P08581 Human
Alternative names: AUTS9 c met D249 Hepatocyte growth factor receptor HGF HGF receptor HGF/SF receptor HGFR MET Met proto oncogene tyrosine kinase MET proto oncogene, receptor tyrosine kinase Met proto-oncogene (hepatocyte growth factor receptor) Met proto-oncogene Met protooncogene MET_HUMAN Oncogene MET Par4 Proto-oncogene c-Met RCCP2 Scatter factor receptor SF receptor Tyrosine-protein kinase Met
Images
ET7110-14_1.jpg Fig1: Western blot analysis of Phospho-Met (Y1349) on different lysates with Rabbit anti-Phospho-Met (Y1349) antibody (ET7110-14) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 156 kDa
Observed band size: 156 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7110-14_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Phospho-Met (Y1349) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-14_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Phospho-Met (Y1349) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-14_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human rectum tissue using anti-Phospho-Met (Y1349) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.