NEK6 Recombinant Rabbit Monoclonal Antibody [JE51-72]
cat.: ET7110-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE51-72 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NEK6 aa 170-313 / 313. |
| Positive control: | THP-1 cell lysate, Wild-type 293T whole cell lysate. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule, Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q9HC98 Human |
| Alternative names: | Highly similar to cell cycle protein kinase CDC5/MSD2 NEK6 NEK6_HUMAN Never in mitosis A-related kinase 6 NIMA (Never In Mitosis Gene A) Related Kinase 6 NimA related protein kinase 6 NimA-related protein kinase 6 Protein kinase SID6 1512 Protein kinase SID6-1512 Putative serine threonine protein kinase Serine/threonine protein kinase Nek6 Serine/threonine-protein kinase Nek6 SID6 1512 SID61512 |
Images
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Fig1: Western blot analysis of NEK6 on THP-1 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
All lanes: Western blot analysis of NEK6 with anti-NEK6 antibody[JE51-72] (ET7110-15) at 1/1,000 dilution. Lane 1: Wild-type 293T whole cell lysate (30 µg). Lane 2: NEK6 knockout 293T whole cell lysate (30 µg). ET7110-15 was shown to specifically react with NEK6 in wild-type 293T cells. No band was observed when NEK6 knockout sample was tested. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7110-15, 1/1,000) was used in 5% BSA at room temperature for 2 hours. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.