NEK7 Recombinant Rabbit Monoclonal Antibody [JE51-70]
cat.: ET7110-16
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE51-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within internal sequence of Human NEK7aa 200-302 / 302. |
| Positive control: | NIH/3T3 cell lysate, Jurkat cell lysate, rat brain tissue, human liver carcinoma tissue, human stomach tissue, mouse skeletal muscle tissue, A549. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule, Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:800 1:50-1:100 |
| Uniprot #: | SwissProt: Q8TDX7 Human | Q9ES74 Mouse | D3ZBE5 Rat |
| Alternative names: | EC 2.7.11.1 NEK7 NEK7_HUMAN Never in mitosis A-related kinase 7 Never in mitosis gene a-related kinase 7 NIMA (never in mitosis gene a) related kinase 7 NIMA (never in mitosis gene a)-related expressed kinase 7 NimA related protein kinase 7 NIMA-related kinase 7 NimA-related protein kinase 7 Serine/threonine-protein kinase Nek7 |
Images
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Fig1:
Western blot analysis of NEK7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Jurkat cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-NEK7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-16, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-NEK7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-16, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-NEK7 antibody (ET7110-16) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-16) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-NEK7 antibody (ET7110-16) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-16) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of NEK7 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-16, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.