NeuroD2 Recombinant Rabbit Monoclonal Antibody [JE50-57]
cat.: ET7110-17
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | JE50-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NeuroD2 aa 260-382 / 382. |
| Positive control: | Rat brain tissue, mouse brain tissue, mouse hippocampus tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P WB |
1:200-1:1,000 1:500 |
| Uniprot #: | SwissProt: Q15784 Human | Q62414 Mouse | Q63689 Rat |
| Alternative names: | bHLHa1 class A basic helix loop helix protein 1 Class A basic helix-loop-helix protein 1 NDF2_HUMAN NDR2 NDRF neuroD related factor NeuroD-related factor NeuroD2 neurogenic basic helix loop helix protein neurogenic differentiation 2 Neurogenic differentiation factor 2 neuronal differentiation 2 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-NeuroD2 antibody (ET7110-17) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NeuroD2 antibody (ET7110-17) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-NeuroD2 antibody (ET7110-17) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.