NeuroD2 Recombinant Rabbit Monoclonal Antibody [JE50-57]
cat.: ET7110-17
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat, Human
Applications: IHC-P, WB
Clonality: Monoclonal
Clone number: JE50-57
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 41 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human NeuroD2 aa 260-382 / 382.
Positive control: Rat brain tissue, mouse brain tissue, mouse hippocampus tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  IHC-P
  WB

1:200-1:1,000
1:500
Uniprot #: SwissProt: Q15784 Human | Q62414 Mouse | Q63689 Rat
Alternative names: bHLHa1 class A basic helix loop helix protein 1 Class A basic helix-loop-helix protein 1 NDF2_HUMAN NDR2 NDRF neuroD related factor NeuroD-related factor NeuroD2 neurogenic basic helix loop helix protein neurogenic differentiation 2 Neurogenic differentiation factor 2 neuronal differentiation 2
Images
ET7110-17_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-NeuroD2 antibody (ET7110-17) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-17_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NeuroD2 antibody (ET7110-17) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-17_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-NeuroD2 antibody (ET7110-17) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.