NUDC Recombinant Rabbit Monoclonal Antibody [JE50-74]
cat.: ET7110-18
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE50-74
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human NUDC aa 207-331 / 331.
Positive control: Mouse testis tissue lysate, rat brain tissue lysate, rat testis tissue lysate, K562 cell lysate, PC-3M cell lysate, U937 cell lysate, human tonsil tissue, human colon carcinoma tissue, human gastric carcinoma tissue, mouse brain tissue, rat brain tissue, K-562.
Subcellular location: Cytoplasm, cytoskeleton, Nucleus, spindle, Midbody.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500-1:5,000
1:100-1:500
1:100
1:1,000
Uniprot #: SwissProt: Q9Y266 Human | O35685 Mouse | Q63525 Rat
Alternative names: HNUDC MNUDC MNUDC protein NPD011 Nuclear distribution C homolog Nuclear distribution gene C (A.nidulans) homolog Nuclear distribution gene C homolog Nuclear distribution gene C homolog (A. nidulans) Nuclear distribution protein C homolog Nuclear migration protein nudC nudC NudC nuclear distribution protein NUDC_HUMAN OTTHUMP00000004405 SIG 92 SIG92
Images
ET7110-18_1.jpg Fig1: Western blot analysis of NUDC on different lysates with Rabbit anti-NUDC antibody (ET7110-18) at 1/5,000 dilution.

Lane 1: Mouse testis tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Rat testis tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 38 kDa
Observed band size: 45 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-18) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7110-18_2.jpg Fig2: Western blot analysis of NUDC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysates
Lane 2: PC-3M cell lysates
Lane 3: U937 cell lysates
ET7110-18_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NUDC antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-18, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-18_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-NUDC antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-18, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-18_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-NUDC antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-18, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-18_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NUDC antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-18, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-18_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NUDC antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-18, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-18_8.jpg Fig8: Immunocytochemistry analysis of K-562 cells labeling NUDC with Rabbit anti-NUDC antibody (ET7110-18) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUDC antibody (ET7110-18) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7110-18_9.jpg Fig9: Flow cytometric analysis of K-562 cells labeling NUDC.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7110-18, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.