ET7110-19

PSMA2 Recombinant Rabbit Monoclonal Antibody [JE51-51]

cat.: ET7110-19

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Green monkey
Applications:	WB, IHC-P
Clonality:	Monoclonal
Clone number:	JE51-51

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 26 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human PSMA2 aa 1-140 / 234.
Positive control:	COS-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HepG2 cell lysate, 293T cell lysate, Hela cell lysate, JAR cell lysate, mouse stomach tissue, rat stomach tissue, human small intestine tissue, human liver carcinoma tissue, human breast carcinoma tissue.
Subcellular location:	Cytoplasm, Nucleus, Proteasome.
Recommended Dilutions: WB IHC-P	1:5,000 1:200-1:1,000
Uniprot #:	SwissProt: P25787 Human \| P49722 Mouse \| P17220 Rat
Alternative names:	HC3 Macropain subunit C3 MU Multicatalytic endopeptidase complex subunit C3 PMSA2 Proteasome (prosome macropain) subunit alpha type 2 Proteasome alpha 2 subunit Proteasome component C3 Proteasome subunit alpha type 2 Proteasome subunit alpha type-2 Proteasome subunit HC3 PSA2_HUMAN PSC2 PSC3 PSMA 2 psmA2

Images

Fig1: Western blot analysis of PSMA2 on different lysates with Rabbit anti-PSMA2 antibody (ET7110-19) at 1/2,000 dilution.

Lane 1: COS-1 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 26 kDa
Observed band size: 26 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-19) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of PSMA2 on different lysates with Rabbit anti-PSMA2 antibody (ET7110-19) at 1/5,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: JAR (Human placental choriocarcinoma cell) cell lysate
Lane 3: 293T (Human embryonic kidney cell) cell lysate
Lane 4: Hep G2 (Human hepatocellular carcinoma cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 25 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7110-19, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 26 kDa
Observed band size: 26 kDa

	Fig3: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-PSMA2 antibody (ET7110-19) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7110-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-PSMA2 antibody (ET7110-19) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7110-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-PSMA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7110-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PSMA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7110-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PSMA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7110-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.