PSMA3 Recombinant Rabbit Monoclonal Antibody [JE51-59]
cat.: ET7110-20
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE51-59
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PSMA3 aa 1-189 / 255.
Positive control: HL-60 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, SW620, rat large intestine tissue, human liver tissue, human breast tissue, human placenta tissue.
Subcellular location: Cytoplasm, Nucleus, Proteasome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:5,000
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: P25788 Human | O70435 Mouse | P18422 Rat
Alternative names: HC8 Macropain subunit C8 MGC12306 MGC32631 Multicatalytic endopeptidase complex subunit C8 Proteasome (prosome macropain) subunit alpha type 3 Proteasome alpha 3 subunit Proteasome component C8 Proteasome subunit alpha type 3 Proteasome subunit alpha type-3 Proteasome subunit C8 PSA3_HUMAN PSC8 psmA3
Images
ET7110-20_1.jpg Fig1: Western blot analysis of PSMA3 on different lysates with Rabbit anti-PSMA3 antibody (ET7110-20) at 1/5,000 dilution.

Lane 1: HL-60 (Human acute promyelocytic leukemia cell)
Lane 2: NIH/3T3 (Mouse fibroblast)
Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated))

Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7110-20, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 28 kDa
Observed band size: 28 kDa
ET7110-20_2.jpg Fig2: ICC staining of PSMA3 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7110-20_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-PSMA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-20_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PSMA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-20_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PSMA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-20_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PSMA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.