TIMM50 (Tim50) Recombinant Rabbit Monoclonal Antibody [JE51-61]
cat.: ET7110-24
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE51-61
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 40 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human TIM50 aa 1-109 / 353.
Positive control: HeLa cell lysate, K-562 cell lysate, NIH:OVCAR-3 cell lysate, PANC-1 cell lysate, human kidney tissue, human liver tissue, mouse liver tissue, rat liver tissue.
Subcellular location: Mitochondrion inner membrane, nucleus speckle.
Recommended Dilutions:
  WB
  IHC-P
1:5,000
1:50-1:100
Uniprot #: SwissProt: Q3ZCQ8 Human | Q9D880 Mouse
Entrez Gene: 685725 Rat
Alternative names: Homolog of yeast Tim50 Import inner membrane translocase subunit TIM 50 Import inner membrane translocase subunit TIM50 Import inner membrane translocase subunit TIM50 mitochondrial MGC102733 Mitochondrial import inner membrane translocase subunit TIM50 TIM 50 TIM 50L TIM50 Tim50 like protein TIM50_HUMAN TIM50L TIMM 50 TIMM50 translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) Translocase of inner mitochondrial membrane 50 homolog (yeast) Translocase of inner mitochondrial membrane 50 homolog Translocase of inner mitochondrial membrane 50, yeast, homolog of
Images
ET7110-24_1.jpg Fig1: Western blot analysis of TIMM50 (Tim50) on different lysates with Rabbit anti-TIMM50 (Tim50) antibody (ET7110-24) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: NIH:OVCAR-3 cell lysate (20 µg/Lane)
Lane 4: PANC-1 cell lysate (20 µg/Lane)

Predicted band size: 40 kDa
Observed band size: 37 kDa

Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-24) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7110-24_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TIMM50 (Tim50) antibody (ET7110-24) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-24_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-TIMM50 (Tim50) antibody (ET7110-24) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-24_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-TIMM50 (Tim50) antibody (ET7110-24) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-24_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-TIMM50 (Tim50) antibody (ET7110-24) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.