TIMM50 (Tim50) Recombinant Rabbit Monoclonal Antibody [JE51-61]
cat.: ET7110-24
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE51-61
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 40 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human TIM50 aa 1-109 / 353.
Positive control: A549 cell lysate, K562 cell lysate, MCF-7 cell lysate, human liver carcinoma tissue, human colon carcinoma tissue, human breast carcinoma tissue, human liver tissue, human kidney tissue.
Subcellular location: Mitochondrion inner membrane, nucleus speckle.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: Q3ZCQ8 Human | Q9D880 Mouse
Alternative names: Homolog of yeast Tim50 Import inner membrane translocase subunit TIM 50 Import inner membrane translocase subunit TIM50 Import inner membrane translocase subunit TIM50 mitochondrial MGC102733 Mitochondrial import inner membrane translocase subunit TIM50 TIM 50 TIM 50L TIM50 Tim50 like protein TIM50_HUMAN TIM50L TIMM 50 TIMM50 translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) Translocase of inner mitochondrial membrane 50 homolog (yeast) Translocase of inner mitochondrial membrane 50 homolog Translocase of inner mitochondrial membrane 50, yeast, homolog of
Images
ET7110-24_1.jpg Fig1: Western blot analysis of TIMM50 (Tim50) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: K562 cell lysate
Lane 3: MCF-7 cell lysate
ET7110-24_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-TIMM50 (Tim50) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-24_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-TIMM50 (Tim50) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-24_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-TIMM50 (Tim50) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-24_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-TIMM50 (Tim50) antibody (ET7110-24) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-24_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TIMM50 (Tim50) antibody (ET7110-24) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.