WAPL Recombinant Rabbit Monoclonal Antibody [JE51-75]
cat.: ET7110-25
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE51-75
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 133 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human WAPL aa 1-118 / 1,190.
Positive control: 293T cell lysate, Jurkat cell lysate, MDA-MB-231 cell lysate, C2C12 cell lysate, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, human small intestine tissue, human pancreas tissue, human lung carcinoma tissue, human colon carcinoma tissue.
Subcellular location: Nucleus, Chromosome, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
1:2,000
1:50-1:500
Uniprot #: SwissProt: Q7Z5K2 Human | Q65Z40 Mouse
Entrez Gene: 290577 Rat
Alternative names: FOE Friend of EBNA2 (Epstein Barr virus nuclear protein 2) Friend of EBNA2 protein KIAA0261 OTTHUMP00000020004 WAPAL WAPL WAPL_HUMAN Wings apart like homolog Wings apart like homolog (Drosophila) Wings apart-like protein homolog
Images
ET7110-25_1.jpg Fig1: Western blot analysis of WAPL on different lysates with Rabbit anti-WAPL antibody (ET7110-25) at 1/2,000 dilution.

Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (20 µg/Lane)

Predicted band size: 133 kDa
Observed band size: 150 kDa

Exposure time: 35 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-25) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7110-25_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-WAPL antibody (ET7110-25) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-25) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-25_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-WAPL antibody (ET7110-25) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-25) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-25_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-WAPL antibody (ET7110-25) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-25) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-25_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-WAPL antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-25, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-25_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-WAPL antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-25, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-25_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-WAPL antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-25, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-25_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-WAPL antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-25, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.