Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE52-72 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 162 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human WRN aa 1,260-1,432 / 1,432. |
Positive control: | K562 cell lysates. |
Subcellular location: | Nucleolus, nucleus, nucleoplasm. |
Recommended Dilutions:
WB |
1:500-1:1,000 |
Uniprot #: | SwissProt: Q14191 Human |
Alternative names: | DKFZp686C2056 DNA helicase DNA helicase, RecQ like type 3 Exonuclease WRN HGNC 12791 OTTHUMP00000225301 RecQ protein-like 2 RecQ-like type 3 RecQ3 RECQL2 RECQL3 Werner syndrome ATP-dependent helicase Werner syndrome helicase Werner syndrome protein Werner syndrome, RecQ helicase like WRN WRN_HUMAN |
Fig1: Western blot analysis of WRN on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |