WRN Recombinant Rabbit Monoclonal Antibody [JE52-72]
cat.: ET7110-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE52-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 162 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human WRN aa 1,260-1,432 / 1,432. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate. |
| Subcellular location: | Nucleolus, nucleus, nucleoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q14191 Human |
| Alternative names: | DKFZp686C2056 DNA helicase DNA helicase, RecQ like type 3 Exonuclease WRN HGNC 12791 OTTHUMP00000225301 RecQ protein-like 2 RecQ-like type 3 RecQ3 RECQL2 RECQL3 Werner syndrome ATP-dependent helicase Werner syndrome helicase Werner syndrome protein Werner syndrome, RecQ helicase like WRN WRN_HUMAN |
Images
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Fig1:
Western blot analysis of WRN on different lysates with Rabbit anti-WRN antibody (ET7110-26) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.