ERAP1 Recombinant Rabbit Monoclonal Antibody [JE52-36]
cat.: ET7110-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE52-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 107 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ERAP1 aa 833-941 / 941. |
| Positive control: | Human skin tissue lysates, 293T cell lysates, rat bladder tissue, human small intestine tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100-1:400 |
| Uniprot #: | SwissProt: Q9NZ08 Human | Q9JJ22 Rat |
| Alternative names: | A LAP A-LAP Adipocyte derived leucine aminopeptidase Adipocyte-derived leucine aminopeptidase ALAP Aminopeptidase PILS Aminopeptidase regulator of TNFR1 shedding APPILS ARTS 1 ARTS-1 Arts1 Endoplasmic reticulum aminopeptidase 1 Endoplasmic reticulum aminopeptidase associated with antigen processing ERAAP ERAAP1 ERAP 1 Erap1 ERAP1_HUMAN KIAA0525 kiaa0525 protein PILS AP PILS-AP PILSA PILSAP Puromycin insensitive leucyl specific aminopeptidase Puromycin-insensitive leucyl-specific aminopeptidase Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator type 1 tumor necrosis factor receptors shedding aminopeptidase regulator VEGF-induced aminopeptidase |
Images
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Fig1:
All lanes: Western blot analysis of ERAP1 with anti-ERAP1 antibody [JE52-36] (ET7110-29) at 1/500 dilution. Lane 1: Wild-type 293T whole cell lysate. Lane 2: ERAP1 knockout 293T whole cell lysate. ET7110-29 was shown to specifically react with ERAP1 in wild-type 293T cells. No band was observed when ERAP1 knockout sample was tested. Wild-type and ERAP1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-ERAP1 antibody (ET7110-29, 1/500) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2:
Western blot analysis of ERAP1 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 107 kDa Observed band size: 107 kDa |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-ERAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-29, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ERAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-29, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.