ERAP1 Recombinant Rabbit Monoclonal Antibody [JE52-36]
cat.: ET7110-29
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE52-36
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 107 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human ERAP1 aa 833-941 / 941.
Positive control: A431 cell lysate, PC-12 cell lysate, Mouse heart tissue lysate, Rat liver tissue lysate, human placenta tissue.
Subcellular location: Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IHC-P
1:1,000
1:1,000
Uniprot #: SwissProt: Q9NZ08 Human | Q9EQH2 Mouse | Q9JJ22 Rat
Alternative names: A LAP A-LAP Adipocyte derived leucine aminopeptidase Adipocyte-derived leucine aminopeptidase ALAP Aminopeptidase PILS Aminopeptidase regulator of TNFR1 shedding APPILS ARTS 1 ARTS-1 Arts1 Endoplasmic reticulum aminopeptidase 1 Endoplasmic reticulum aminopeptidase associated with antigen processing ERAAP ERAAP1 ERAP 1 Erap1 ERAP1_HUMAN KIAA0525 kiaa0525 protein PILS AP PILS-AP PILSA PILSAP Puromycin insensitive leucyl specific aminopeptidase Puromycin-insensitive leucyl-specific aminopeptidase Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator type 1 tumor necrosis factor receptors shedding aminopeptidase regulator VEGF-induced aminopeptidase
Images
ET7110-29_1.jpg Fig1: All lanes: Western blot analysis of ERAP1 with anti-ERAP1 antibody [JE52-36] (ET7110-29) at 1/500 dilution.

Lane 1: Wild-type 293T whole cell lysate.
Lane 2: ERAP1 knockout 293T whole cell lysate.

ET7110-29 was shown to specifically react with ERAP1 in wild-type 293T cells. No band was observed when ERAP1 knockout sample was tested. Wild-type and ERAP1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-ERAP1 antibody (ET7110-29, 1/500) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
ET7110-29_2.jpg Fig2: Western blot analysis of ERAP1 on different lysates with Rabbit anti-ERAP1 antibody (ET7110-29) at 1/1,000 dilution.

Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Lane 3: Mouse heart tissue lysate (40 µg/Lane)
Lane 4: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 107 kDa
Observed band size: 107 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7110-29_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ERAP1 antibody (ET7110-29) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.