DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50]
cat.: ET7110-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE52-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DBF4 aa 1-107 / 674. |
| Positive control: | 293T cell lysate, Hela cell lysate, SW480 cell lysate, Jurkat cell lysate, F9. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UBU7 Human | Q9QZ41 Mouse |
| Alternative names: | Activator of S phase kinase ASK CHIF Chiffon homolog A DBF4 DBF4 homolog (S. cerevisiae) DBF4 homolog DBF4 type zinc finger containing protein 1 DBF4 zinc finger A DBF4 zinc finger DBF4, S. cerevisiae, homolog of DBF4-type zinc finger-containing protein 1 DBF4A DBF4A_HUMAN Dumbbell former 4, S. cerevisiae, homolog of OTTHUMP00000161661 Protein DBF4 homolog A ZDBF1 Zinc finger, DBF-type containing 1 |
Images
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Fig1:
Western blot analysis of DBF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: Hela cell lysate Lane 3: SW480 cell lysate Lane 4: Jurkat cell lysate |
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Fig2: Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.