DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50]
cat.: ET7110-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE52-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DBF4 aa 1-107 / 674. |
| Positive control: | 293T cell lysate, Hela cell lysate, SW480 cell lysate, Jurkat cell lysate, F9. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UBU7 Human | Q9QZ41 Mouse |
| Alternative names: | Activator of S phase kinase ASK CHIF Chiffon homolog A DBF4 DBF4 homolog (S. cerevisiae) DBF4 homolog DBF4 type zinc finger containing protein 1 DBF4 zinc finger A DBF4 zinc finger DBF4, S. cerevisiae, homolog of DBF4-type zinc finger-containing protein 1 DBF4A DBF4A_HUMAN Dumbbell former 4, S. cerevisiae, homolog of OTTHUMP00000161661 Protein DBF4 homolog A ZDBF1 Zinc finger, DBF-type containing 1 |
Images
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Fig1:
Western blot analysis of DBF4 on different lysates with Rabbit anti-DBF4 antibody (ET7110-31) at 1/5,000 dilution. Lane 1: 293T (Human embryonic kidney cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: SW480 (Human colorectal zdenocarcinoma cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7110-31, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 76.9 kDa Observed band size: 76.9 kDa |
|
Fig2: Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.