SENP2 Recombinant Rabbit Monoclonal Antibody [JE52-69]
cat.: ET7110-33
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE52-69
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 68 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SENP2 aa 280-420 / 589.
Positive control: 293 cell lysates, human colon tissue, human breast tissue, human kidney tissue.
Subcellular location: Cytoplasm, Membrane, Nuclear pore complex, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: Q9HC62 Human
Alternative names: Axam2 DKFZp762A2316 KIAA1331 Senp2 SENP2_HUMAN Sentrin specific protease 2 Sentrin-specific protease 2 Sentrin/SUMO specific protease SENP2 Sentrin/SUMO-specific protease SENP2 SMT3 specific isopeptidase 2 SMT3-specific isopeptidase 2 Smt3ip2 SUMO1/sentrin/SMT3 specific peptidase 2
Images
ET7110-33_1.jpg Fig1: Western blot analysis of SENP2 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7110-33_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SENP2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-33_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SENP2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-33_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SENP2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.