WNT5B Recombinant Rabbit Monoclonal Antibody [JE53-67]
cat.: ET7110-34
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE53-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human WNT5B aa 260-359 / 359. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, LOVO. |
| Subcellular location: | Extracellular matrix, Secreted. |
| Recommended Dilutions:
WB FC |
1:5,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9H1J7 Human |
| Alternative names: | MGC2648 Protein Wnt-5b Wingless type MMTV integration site family, member 5B WNT 5B WNT 5B protein Wnt family member 5B Wnt5b WNT5B protein WNT5B_HUMAN |
Images
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Fig1:
Western blot analysis of WNT5B on different lysates with Rabbit anti-WNT5B antibody (ET7110-34) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7110-34, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 45 kDa |
|
Fig2: Flow cytometric analysis of WNT5B was done on LOVO cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-34, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.