SLC22A3 Recombinant Rabbit Monoclonal Antibody [JE53-70]
cat.: ET7110-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE53-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SLC22A3 aa 281-470 / 556. |
| Positive control: | Human placenta tissue lysates, human colon carcinoma tissue, A549. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1000-1:5,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O75751 Human |
| Alternative names: | EMT EMT organic cation transporter 3 EMTH Extraneuronal monoamine transporter OCT 3 OCT3 Orct 3 Orct3 Organic cation transporter 3 S22A3_HUMAN SLC22 A3 SLC22A 3 Slc22a3 Solute carrier family 22 (extraneuronal monoamine transporter) member 3 Solute carrier family 22 (organic cation transporter) member 3 Solute carrier family 22 member 3 |
Images
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Fig1:
Western blot analysis of SLC22A3 on human placenta tissue lysates with Rabbit anti-SLC22A3 antibody (ET7110-35) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-35) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-SLC22A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-35, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of SLC22A3 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.