SPAK Recombinant Rabbit Monoclonal Antibody [JE53-64]
cat.: ET7110-37
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE53-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SPAK aa 361-545 / 545. |
| Positive control: | Jurkat cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat brain tissue lysate, Rat testis tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue, human prostate carcinoma tissue, human pancreas tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q9UEW8 Human | Q9Z1W9 Mouse | O88506 Rat |
| Alternative names: | DCHT DKFZp686K05124 OTTHUMP00000165175 PASK Proline alanine rich STE20 related kinase Serine threonine kinase 39 (STE20/SPS1 homolog yeast) Serine threonine kinase 39 Serine/threonine protein kinase 39 Serine/threonine-protein kinase 39 Small intestine SPAK like kinase SPAK Ste 20 related kinase Ste-20-related kinase Ste20 like protein kinase STE20/SPS1 homolog STE20/SPS1 related proline alanine rich protein kinase STE20/SPS1-related proline-alanine-rich protein kinase STK 39 Stk39 STK39_HUMAN |
Images
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Fig1:
Western blot analysis of SPAK on different lysates with Rabbit anti-SPAK antibody (ET7110-37) at 1/5,000 dilution. Lane 1: Jurkat cell lysate (15 µg/Lane) Lane 2: Mouse brain tissue lysate (15 µg/Lane) Lane 3: Mouse testis tissue lysate (15 µg/Lane) Lane 4: Rat brain tissue lysate (15 µg/Lane) Lane 5: Rat testis tissue lysate (15 µg/Lane) Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 13 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-37) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SPAK antibody (ET7110-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SPAK antibody (ET7110-37) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-37) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SPAK antibody (ET7110-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-SPAK antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-SPAK antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.