HMGN2 Recombinant Rabbit Monoclonal Antibody [JE53-85]
cat.: ET7110-38
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE53-85
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 9 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human HMGN2 aa 1-90 / 90.
Positive control: Daudi cell lysates, A431, MG-63, human lung tissue, human thyroid tissue, human colon tissue, human pancreas tissue.
Subcellular location: Nucleus, cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: P05204 Human
Alternative names: High mobility group (nonhistone chromosomal) protein 17 high mobility group nucleosomal binding domain 2 High mobility group nucleosome-binding domain-containing protein 2 High mobility group protein N2 HMG17 HMGN2 HMGN2_HUMAN MGC5629 Non histone chromosomal protein HMG 17 Non-histone chromosomal protein HMG-17 Nonhistone chromosomal protein HMG 17 nonhistone chromosomal protein hmg-17
Images
ET7110-38_1.jpg Fig1: Western blot analysis of HMGN2 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7110-38_2.jpg Fig2: ICC staining of HMGN2 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7110-38_3.jpg Fig3: ICC staining of HMGN2 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7110-38_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-HMGN2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-38, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-38_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HMGN2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-38, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-38_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-HMGN2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-38, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-38_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-HMGN2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-38, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.