ABI2 Recombinant Rabbit Monoclonal Antibody [JE53-89]
cat.: ET7110-42
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE53-89
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 56 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human ABI2 aa 312-411 / 513.
Positive control: K-562 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, human lung tissue, mouse testis tissue, PC-12.
Subcellular location: Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1,000
1:50-1:200
1:100
1:1,000
Uniprot #: SwissProt: Q9NYB9 Human | P62484 Mouse
Entrez Gene: 286928 Rat
Alternative names: Abelson interactor 2 ABI 2 Abi-2 ABI2 ABI2_HUMAN ABI2B Abl binding protein 3 Abl interacting protein 1 (SH3 containing protein) Abl interactor 2 Abl interactor protein 2b Abl-binding protein 3 AblBP3 AIP 1 Arg binding protein 1 Arg protein tyrosine kinase binding protein Arg-binding protein 1 ArgBP1 ARGBPIA ArgBPIB OTTHUMP00000163783 OTTHUMP00000163784 OTTHUMP00000206182 OTTHUMP00000206183 OTTHUMP00000206184 OTTHUMP00000206185 SSH3BP2
Images
ET7110-42_1.jpg Fig1: Western blot analysis of ABI2 on different lysates with Rabbit anti-ABI2 antibody (ET7110-42) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 56 kDa
Observed band size: 73 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7110-42_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-ABI2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-42_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ABI2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-42_4.jpg Fig4: Immunocytochemistry analysis of PC-12 cells labeling ABI2 with Rabbit anti-ABI2 antibody (ET7110-42) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ABI2 antibody (ET7110-42) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7110-42_5.jpg Fig5: Flow cytometric analysis of PC-12 cells labeling ABI2.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7110-42, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.