ABI2 Recombinant Rabbit Monoclonal Antibody [JE53-89]
cat.: ET7110-42
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE53-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ABI2 aa 312-411 / 513. |
| Positive control: | MCF-7 cell lysates, human lung tissue, mouse testis tissue. |
| Subcellular location: | Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9NYB9 Human | P62484 Mouse |
| Alternative names: | Abelson interactor 2 ABI 2 Abi-2 ABI2 ABI2_HUMAN ABI2B Abl binding protein 3 Abl interacting protein 1 (SH3 containing protein) Abl interactor 2 Abl interactor protein 2b Abl-binding protein 3 AblBP3 AIP 1 Arg binding protein 1 Arg protein tyrosine kinase binding protein Arg-binding protein 1 ArgBP1 ARGBPIA ArgBPIB OTTHUMP00000163783 OTTHUMP00000163784 OTTHUMP00000206182 OTTHUMP00000206183 OTTHUMP00000206184 OTTHUMP00000206185 SSH3BP2 |
Images
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Fig1: Western blot analysis of ABI2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-ABI2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ABI2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.