DP1 Recombinant Rabbit Monoclonal Antibody [JE52-55]
cat.: ET7110-43
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE52-55 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DP1 aa 301-410 / 410. |
| Positive control: | SiHa cell lysate, SK-OV-3 cell lysate, HeLa cell lysate, C2C12 cell lysate, rat large intestine tissue, human lung carcinoma tissue, mouse colon tissue, HeLa, C2C12. |
| Subcellular location: | Nucleus, cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: Q14186 Human | Q08639 Mouse | G3V8H9 Rat |
| Alternative names: | DP 1 DP1 DRTF1 DRTF1-polypeptide 1 E2F dimerization partner 1 E2F related transcription factor TFDP1 TFDP1_HUMAN Transcription factor Dp-1 Transcription factor sequence-specific, DRTF1 |
Images
|
Fig1:
Western blot analysis of DP1 on different lysates with Rabbit anti-DP1 antibody (ET7110-43) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: C2C12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 49 kDa Exposure time: Lane 1: 30 seconds; Lane 2-4: 7 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-43) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-DP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-DP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-DP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Flow cytometric analysis of HeLa cells labeling DP1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7110-43, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig6:
Flow cytometric analysis of C2C12 cells labeling DP1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7110-43, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.