DP1 Recombinant Rabbit Monoclonal Antibody [JE52-55]
cat.: ET7110-43
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE52-55
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human DP1 aa 301-410 / 410.
Positive control: SiHa cell lysate, SH-SY5Y cell lysate, C2C12 cell lysates, mouse thymus tissue lysate, rat large intestine tissue, human lung carcinoma tissue, mouse colon tissue, THP-1.
Subcellular location: Nucleus, cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q14186 Human | Q08639 Mouse | G3V8H9 Rat
Alternative names: DP 1 DP1 DRTF1 DRTF1-polypeptide 1 E2F dimerization partner 1 E2F related transcription factor TFDP1 TFDP1_HUMAN Transcription factor Dp-1 Transcription factor sequence-specific, DRTF1
Images
ET7110-43_1.jpg Fig1: Western blot analysis of DP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-43, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: Mouse thymus tissue lysate
ET7110-43_2.jpg Fig2: Western blot analysis of DP1 on C2C12 cell lysates with Rabbit anti-DP1 antibody (ET7110-43) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-43) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7110-43_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-DP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-43_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-DP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-43_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-DP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-43_6.jpg Fig6: Flow cytometric analysis of DP1 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-43, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.