Anti-MUM1 antibody [JE52-60]
cat.: ET7110-44
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE52-60
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 52 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MUM1 aa 1-100.
Positive control: Human tonsil tissue, human colon tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: Q15306 Human
Alternative names: "Interferon regulatory factor 4 IRF 4 IRF-4 Irf4 IRF4_HUMAN LSIRF Lymphocyte specific interferon regulatory factor Lymphocyte specific IRF Lymphocyte-specific interferon regulatory factor Multiple myeloma oncogene 1 MUM 1 MUM1 NF EM5 NF-EM5 NFEM5 PU.1 interaction partner Sfpi1/PU.1 interaction partner Transcriptional activator PIP "
Images
ET7110-44_1.jpg Fig1: Western blot analysis of MUM1 on SKBR3 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7110-44_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MUM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-44_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MUM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.