SIRT1 Recombinant Rabbit Monoclonal Antibody [JE11-04]
cat.: ET7110-49
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE11-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 82 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C terminal human SIRT1. |
| Positive control: | Jurkat cell lysate, HepG2 cell lysate, Hela cell lysate, Hela. |
| Subcellular location: | Cytoplasm, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1000-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q96EB6 Human |
| Alternative names: | 75SirT1 hSIR2 hSIRT1 HST2 HST2, S. cerevisiae, homolog of NAD dependent deacetylase sirtuin 1 NAD dependent protein deacetylase sirtuin 1 NAD-dependent deacetylase sirtuin-1 OTTHUMP00000198111 OTTHUMP00000198112 Regulatory protein SIR2 homolog 1 SIR1_HUMAN SIR2 SIR2 like 1 SIR2 like protein 1 SIR2, S.cerevisiae, homolog-like 1 SIR2-like protein 1 SIR2ALPHA SIR2L1 Sirt1 SirtT1 75 kDa fragment Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) Sirtuin 1 Sirtuin type 1 |
Images
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Fig1:
Western blot analysis of SIRT1 on different lysates with Rabbit anti-SIRT1 antibody (ET7110-49) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SIRT1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa Observed band size: 120 kDa Exposure time: 78 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-49) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SIRT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: HepG2 cell lysate Lane 3: Hela cell lysate |
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Fig3: ICC staining of SIRT1 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-49, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.