Anti-MGMT antibody [JE31-37]
cat.: ET7110-52
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE31-37
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL
Purification: Protein A affinity purified.
Molecular weight: 22 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N terminal Human MGMT.
Positive control: MCF-7 cell lysate, Daudi cell lysate, SiHa cell lysate, human liver carcinoma tissue, human thyroid tissue, human breast tissue, human breast carcinoma tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: P16455 Human
Alternative names: 6 O methylguanine DNA methyltransferase 6-O-methylguanine-DNA methyltransferase Agat AGT AI267024 EC 2.1.1.63 Methylated DNA protein cysteine methyltransferase Methylated-DNA--protein-cysteine methyltransferase Methylguanine DNA methyltransferase MGC107020 MGMT MGMT_HUMAN O 6 methylguanine DNA alkyltransferase O 6 methylguanine DNA methyltransferase O-6-methylguanine-DNA methyltransferase O-6-methylguanine-DNA-alkyltransferase
Images
ET7110-52_1.jpg Fig1: Western blot analysis of MGMT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Daudi cell lysate
Lane 3: SiHa cell lysate
ET7110-52_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-MGMT antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-52, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-52_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-MGMT antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-52, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-52_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MGMT antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-52, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-52_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MGMT antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-52, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.