Villin1 Recombinant Rabbit Monoclonal Antibody [JE39-08]
cat.: ET7110-55
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE39-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within |
| Positive control: | Caco-2 cell lysate, COLO205 cell lysate, HepG2 cell lysate, AGS cell lysate, human colon tissue, human colon cancer tissue, human kidney tissue. |
| Subcellular location: | Cell projection, Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P IP IF-Tissue |
1:5,000 1:1,000 1-2μg/sample 1:200 |
| Uniprot #: | SwissProt: P09327 Human |
| Alternative names: | D2S1471 OTTHUMP00000164145 VIL VIL1 VILI_HUMAN Villin 1 Villin-1 Villin1 |
Images
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Fig1:
Western blot analysis of Villin1 on different lysates with Rabbit anti-Villin1 antibody (ET7110-55) at 1/5,000 dilution. Lane 1: Caco-2 cell lysate (20 µg/Lane) Lane 2: COLO205 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: AGS cell lysate (20 µg/Lane) Lane 5: PANC-1 cell lysate (negative) (20 µg/Lane) Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-55) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Villin1 antibody (ET7110-55) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Villin1 antibody (ET7110-55) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin1 antibody (ET7110-55) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Villin1 was immunoprecipitated from 0.2 mg Caco-2 cell lysate with ET7110-55 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET7110-55 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Caco-2 cell lysate (input) Lane 2: ET7110-55 IP in Caco-2 cell lysate Lane 3: Rabbit IgG instead of ET7110-55 in Caco-2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 6 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.