Villin1 Recombinant Rabbit Monoclonal Antibody [JE39-08]
cat.: ET7110-55
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE39-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Villin1 aa 778-827 / 827. |
| Positive control: | Human stomach tissue lysates, human liver tissue lysates, human liver tissue tissue. |
| Subcellular location: | Cell projection, Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P09327 Human |
| Alternative names: | D2S1471 OTTHUMP00000164145 VIL VIL1 VILI_HUMAN Villin 1 Villin-1 Villin1 |
Images
|
Fig1:
Western blot analysis of Villin1 on human stomach tissue lysates with Rabbit anti-Villin1 antibody (ET7110-55) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 100 kDa Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-55) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Villin1 on human liver tissue lysates lysates with Rabbit anti-Villin1 antibody (ET7110-55) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 100 kDa Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-55) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue tissue with Rabbit anti-Villin1 antibody (ET7110-55) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-55) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.