Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [JE22-64]
cat.: ET7110-61
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE22-64
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 87 kDa (Predicted band size)
Isotype: IgG
Immunogen: Synthetic peptide within Human Integrin beta 3 aa 739-788 / 788.
Positive control: HUVEC cell lysates, human thyroid tissue, human colon carcinoma tissue, human spleen tissue.
Subcellular location: Cell membrane, lamellipodium membrane, postsynaptic cell membrane, focal adhesion, synapse.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: P05106 Human | O54890 Mouse
Alternative names: BDPLT16 BDPLT2 CD 61 CD61 CD61 antigen GP3A GPIIIa GT HPA 1 HPA 4 Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) Integrin beta chain beta 3 Integrin beta-3 ITB3_HUMAN ITG B3 ITGB 3 ITGB3 NAIT platelet fibrinogen receptor Platelet fibrinogen receptor beta subunit Platelet fibrinogen receptor, beta subunit Platelet glycoprotein IIIa Platelet glycoprotein IIIa precursor Platelet membrane glycoprotein IIIa PTP
Images
ET7110-61_1.jpg Fig1: Western blot analysis of Integrin beta 3 on HUVEC cell lysates with Rabbit anti-Integrin beta 3 antibody (ET7110-61) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 87 kDa
Observed band size:120 kDa

Exposure time: 2 minutes;

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-61) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7110-61_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Integrin beta 3 antibody (ET7110-61) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-61) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-61_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Integrin beta 3 antibody (ET7110-61) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-61) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-61_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody (ET7110-61) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-61) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.