Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P |
Clonality: | Monoclonal |
Clone number: | JE19-84 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size 29 kDa. |
Isotype: | IgG |
Immunogen: | Synthetic peptide within C-terminal Human Prostate Specific Antigen. |
Positive control: | Human prostate tissue. |
Subcellular location: | Secreted. |
Recommended Dilutions:
IHC-P |
1:50-1:200 |
Uniprot #: | SwissProt: P07288 Human |
Alternative names: | antigen, prostate-specific APS Gamma seminoprotein Gamma-seminoprotein hK3 Kallikrein 3 Kallikrein related peptidase 3 Kallikrein-3 KLK 3 KLK2A1 Klk3 KLK3_HUMAN P-30 antigen P30 antigen Prostate-specific antigen Psa Semenogelase Seminin |
Fig1:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Prostate Specific Antigen antibody (ET7110-62) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-62) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |