Prostate Specific Antigen Recombinant Rabbit Monoclonal Antibody [JE19-84]
cat.: ET7110-62
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P
Clonality: Monoclonal
Clone number: JE19-84
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size 29 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal Human Prostate Specific Antigen.
Positive control: Human prostate tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  IHC-P

1:50-1:200
Uniprot #: SwissProt: P07288 Human
Alternative names: antigen, prostate-specific APS Gamma seminoprotein Gamma-seminoprotein hK3 Kallikrein 3 Kallikrein related peptidase 3 Kallikrein-3 KLK 3 KLK2A1 Klk3 KLK3_HUMAN P-30 antigen P30 antigen Prostate-specific antigen Psa Semenogelase Seminin
Images
ET7110-62_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Prostate Specific Antigen antibody (ET7110-62) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-62) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.