CD3 epsilon Recombinant Rabbit Monoclonal Antibody [JE17-74]
cat.: ET7110-63
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE17-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD3 epsilon aa 158-207 / 207. |
| Positive control: | Jurkat cell lysates, human tonsil tissue, human spleen tissue, Jurkat. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P07766 Human |
| Alternative names: | CD3 epsilon CD3e CD3e antigen CD3e antigen epsilon polypeptide (TiT3 complex) CD3E antigen epsilon polypeptide CD3E antigen epsilon subunit CD3e molecule epsilon CD3e molecule epsilon (CD3 TCR complex) CD3e molecule epsilon (CD3-TCR complex) CD3E_HUMAN IMD18 T cell antigen receptor complex epsilon subunit of T3 T cell surface antigen T3/Leu 4 epsilon chain T cell surface glycoprotein CD3 epsilon chain T-cell surface antigen T3/Leu-4 epsilon chain T-cell surface glycoprotein CD3 epsilon chain T3E TCRE |
Images
|
Fig1:
Western blot analysis of CD3 epsilon on Jurkat cell lysates with Rabbit anti-CD3 epsilon antibody (ET7110-63) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size:23 kDa Exposure time: 2 minutes; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-63) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD3 epsilon antibody (ET7110-63) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-63) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD3 epsilon antibody (ET7110-63) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-63) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Flow cytometric analysis of CD3 epsilon was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-63, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.