Beta glucuronidase Recombinant Rabbit Monoclonal Antibody [JE54-34]
cat.: ET7110-66
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE54-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 75 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human beta glucuronidase aa 1-160 / 651. |
| Positive control: | U937 cell lysate, human lung tissue lysate, THP-1 cell lysates, human prostate carcinoma tissue, human kidney tissue, THP-1. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P08236 Human |
| Alternative names: | Ac2-223 asd Beta G1 Beta glucuronidase Beta-G1 Beta-glucuronidase BG BGLR BGLR_HUMAN FLJ39445 Glucuronidase beta Gur Gus Gus-r Gus-s Gus-t Gus-u GUSB Gut MPS7 |
Images
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Fig1:
Western blot analysis of Beta glucuronidase on different lysates with Rabbit anti-Beta glucuronidase antibody (ET7110-66) at 1/500 dilution. Lane 1: U937 cell lysate Lane 2: Human lung tissue lysate(20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-66) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Beta glucuronidase on THP-1 cell lysates with Rabbit anti-Beta glucuronidase antibody (ET7110-66) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-66) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Beta glucuronidase antibody (ET7110-66) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-66) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Beta glucuronidase antibody (ET7110-66) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-66) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Beta glucuronidase was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-66, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.