Syntaxin 1a Recombinant Rabbit Monoclonal Antibody [JE54-37]
cat.: ET7110-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE54-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Syntaxin 1a aa 1-50 / 288. |
| Positive control: | Mouse bone marrow tissue lysates, human cerebellum tissue lysates, rat brain tissue, human kidney tissue, human pancreas tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane, synaptic vesicle membrane, synaptosome; Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q16623 Human | O35526 Mouse | P32851 Rat |
| Alternative names: | HPC 1 Neuron specific antigen HPC1 Neuron-specific antigen HPC-1 OTTHUMP00000174615 OTTHUMP00000174616 OTTHUMP00000174617 OTTHUMP00000174618 P35-1 STX1 STX1A STX1A_HUMAN SYN1A Syntaxin 1A (brain) Syntaxin 1A brain Syntaxin-1A |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Syntaxin 1a antibody (ET7110-68) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-68) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Syntaxin 1a antibody (ET7110-68) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-68) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Syntaxin 1a antibody (ET7110-68) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-68) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Syntaxin 1a was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-68, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Western blot analysis of Syntaxin 1a on different lysates with Rabbit anti-Syntaxin 1a antibody (ET7110-68) at 1/1,000 dilution. Lane 1: Rat cerebellum tissue lysate Lane 2: Mouse cerebellum tissue lysate Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7110-68, 1/1,000 2 hour at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 33.0 kDa Observed band size: 37 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.