Syntaxin 1a Recombinant Rabbit Monoclonal Antibody [JE54-37]
cat.: ET7110-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Syntaxin 1a aa 1-50 / 288. |
| Positive control: | Mouse bone marrow tissue lysates, human cerebellum tissue lysates, rat brain tissue, human kidney tissue, human pancreas tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane, synaptic vesicle membrane, synaptosome; Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: Q16623 Human | O35526 Mouse | P32851 Rat |
| Alternative names: | HPC 1 Neuron specific antigen HPC1 Neuron-specific antigen HPC-1 OTTHUMP00000174615 OTTHUMP00000174616 OTTHUMP00000174617 OTTHUMP00000174618 P35-1 STX1 STX1A STX1A_HUMAN SYN1A Syntaxin 1A (brain) Syntaxin 1A brain Syntaxin-1A |
Images
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Fig1:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET7110-68, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of Syntaxin 1a protein in heart is consistent with the predicted expression pattern. |
|
Fig2:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET7110-68, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of Syntaxin 1a protein in heart is consistent with the predicted expression pattern. |
|
Fig3:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET7110-68, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of Syntaxin 1a protein in heart is consistent with the predicted expression pattern. |
|
Fig4:
Western blot analysis of Syntaxin 1a on different lysates with Rabbit anti-Syntaxin 1a antibody (ET7110-68) at 1/1,000 dilution. Lane 1: Rat cerebellum tissue lysate Lane 2: Mouse cerebellum tissue lysate Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7110-68, 1/1,000 2 hour at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 33.0 kDa Observed band size: 37 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.