MYH7B Recombinant Rabbit Monoclonal Antibody [JE54-44]
cat.: ET7110-69
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat, Mouse, Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE54-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 226 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MYH7B aa 1,710-1,983 / 1,983. |
| Positive control: | Rat heart tissue lysate, Rat skeletal muscle tissue lysate, Mouse skeletal muscle tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: A7E2Y1 Human | A2AQP0 Mouse Entrez Gene: 311570 Rat |
| Alternative names: | Antigen MLAA-21 cardiac muscle beta isoform Myh7b MYH7B_HUMAN Myosin cardiac muscle beta chain Myosin heavy chain 7B Myosin-7B Slow A MYH14 |
Images
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Fig1:
Western blot analysis of MYH7B on different lysates with Rabbit anti-MYH7B antibody (ET7110-69) at 1/1,000 dilution. Lane 1: Rat heart tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lane 3: Mouse skeletal muscle tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 226 kDa Observed band size: 226 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-69) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.