RPL7A Recombinant Rabbit Monoclonal Antibody [JE54-45]
cat.: ET7110-70
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RPL7A aa 1-165 / 266. |
| Positive control: | A431 cell lysate, Jurkat cell lysate, Raji cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, mouse brain tissue. |
| Subcellular location: | Cytosol, cytosolic large ribosomal subunit, nucleolus, nucleus, cytoplasm, focal adhesion, membrane, polysomal ribosome, synapse. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P62424 Human | P12970 Mouse |
| Alternative names: | 60S ribosomal protein L7a L7A MGC103146 PLA X polypeptide PLA-X polypeptide ribosomal protein L7a RL7A_HUMAN RP23-414L19.4 RPL7A SURF 3 SURF-3 SURF3 surfeit 3 Surfeit locus protein 3 Thyroid hormone receptor uncoupling protein TRUP |
Images
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Fig1:
Western blot analysis of RPL7A on different lysates with Rabbit anti-RPL7A antibody (ET7110-70) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-70) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPL7A antibody (ET7110-70) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-70) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.