ET7110-71

GOT2 Recombinant Rabbit Monoclonal Antibody [JE54-46]

cat.: ET7110-71

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Zebrafish
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JE54-46

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 48 kDa.
Isotype:	IgG
Immunogen:	Recombinant protein within Human GOT2 aa 1-139 / 430.
Positive control:	Rat brain tissue lysate, mouse brain tissue lysate, zebrafish tissue lysates, rat smooth muscle tissue, human liver tissue, human colon carcinoma tissue, mouse kidney tissue, SiHa.
Subcellular location:	Cell membrane, mitochondrion matrix.
Recommended Dilutions: WB IHC-P FC	1:1,000-1:2,000 1:50-1:800 1:50-1:100
Uniprot #:	SwissProt: P00505 Human \| P05202 Mouse \| P00507 Rat \| Q7SYK7 DANRE
Alternative names:	AATM_HUMAN AL022787 Aspartate aminotransferase 2 Aspartate aminotransferase Aspartate aminotransferase, mitochondrial Aspartate transaminase 2 ASPATA EC 2.6.1.1 FABP 1 FABP pm FABP-1 FABPpm Fatty acid binding protein Fatty acid-binding protein FLJ40994 Glutamate oxaloacetate transaminase 2 Glutamate oxaloacetate transaminase 2, mitochondrial Glutamate oxaloacetate transaminase, mitochondrial Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) Got 2 GOT2 KAT4 KATIV kynurenine aminotransferase 4 Kynurenine aminotransferase IV kynurenine--oxoglutarate transaminase 4 kynurenine--oxoglutarate transaminase IV mAspAT MGC102129 MGC115763 mitAAT mitochondrial Mitochondrial aspartate aminotransferase OTTMUSP00000017748 Plasma membrane fatty acid binding protein Plasma membrane-associated fatty acid-binding protein Transaminase A

Images

Fig1: Western blot analysis of GOT2 on different lysates with Rabbit anti-GOT2 antibody (ET7110-71) at 1/500 dilution.

Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 48 kDa
Observed band size: 40 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-71) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of GOT2 on zebrafish tissue lysates with Rabbit anti-GOT2 antibody (ET7110-71) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-71) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue with Rabbit anti-GOT2 antibody (ET7110-71) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7110-71) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GOT2 antibody (ET7110-71) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7110-71) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-GOT2 antibody (ET7110-71) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7110-71) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GOT2 antibody (ET7110-71) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7110-71) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Flow cytometric analysis of GOT2 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-71, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.