KLC1 Recombinant Rabbit Monoclonal Antibody [JE54-47]
cat.: ET7110-72
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE54-47 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human KLC1 aa 461-573 / 573. |
| Positive control: | rat brain tissue lysate, Hela cell lysate, MCF-7, RWPE-1, rat testis tissue, human kidney tissue, mouse brain tissue, SH-SY5Y. |
| Subcellular location: | Cytoskeleton, growth cone, cytoplasmic vesicle. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:100 1:50-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q07866 Human | O88447 Mouse | P37285 Rat |
| Alternative names: | Kinesin 2 60/70kDa Kinesin light chain 1 Kinesin2 KLC 1 KLC Klc1 KLC1_HUMAN KNS 2 KNS 2A KNS2 KNS2A Medulloblastoma antigen MU MB 2.50 MGC15245 |
Images
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Fig1:
Western blot analysis of KLC1 on different lysates with Rabbit anti-KLC1 antibody (ET7110-72) at 1/500 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) Lysates/proteins at 10 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7110-72, 1/500 in5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/40,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 65 kDa Observed band size: 65 kDa |
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Fig2:
Western blot analysis of KLC1 on different lysates with Rabbit anti-KLC1 antibody (ET7110-72) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7110-72, 1/500 in5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/40,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 65 kDa Observed band size: 65 kDa |
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Fig3:
Immunocytochemistry analysis of MCF-7 cells labeling KLC1 with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of RWPE-1 cells labeling KLC1 with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-72) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-KLC1 antibody (ET7110-72) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-72) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-KLC1 antibody (ET7110-72) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-72) at 1/400 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8: Flow cytometric analysis of KLC1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-72, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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