KLC1 Recombinant Rabbit Monoclonal Antibody [JE54-47]
cat.: ET7110-72
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE54-47
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 65 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human KLC1 aa 461-573 / 573.
Positive control: Human brain tissue lysate, rat brain tissue lysate, Hela cell lysate, PC-12 cell lysate, MCF-7, RWPE-1, rat testis tissue, human kidney tissue, mouse brain tissue, SH-SY5Y.
Subcellular location: Cytoskeleton, growth cone, cytoplasmic vesicle.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:100
1:50-1:500
1:50-1:100
Uniprot #: SwissProt: Q07866 Human | O88447 Mouse | P37285 Rat
Alternative names: Kinesin 2 60/70kDa Kinesin light chain 1 Kinesin2 KLC 1 KLC Klc1 KLC1_HUMAN KNS 2 KNS 2A KNS2 KNS2A Medulloblastoma antigen MU MB 2.50 MGC15245
Images
ET7110-72_1.jpg Fig1: Western blot analysis of KLC1 on different lysates with Rabbit anti-KLC1 antibody (ET7110-72) at 1/500 dilution.

Lane 1: Human brain tissue lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Lane 3: Hela cell lysate
Lane 4: PC-12 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 65 kDa
Observed band size: 65 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-72) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7110-72_2.jpg Fig2: Immunocytochemistry analysis of MCF-7 cells labeling KLC1 with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI.
ET7110-72_3.jpg Fig3: Immunocytochemistry analysis of RWPE-1 cells labeling KLC1 with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI.
ET7110-72_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-KLC1 antibody (ET7110-72) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-72) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-72_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-KLC1 antibody (ET7110-72) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-72) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-72_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-KLC1 antibody (ET7110-72) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-72) at 1/400 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-72_7.jpg Fig7: Flow cytometric analysis of KLC1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-72, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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