Anti-DOK1 antibody [JE54-52]
cat.: ET7110-75
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE54-52
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 52 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to Human DOK1.
Positive control: K562 cell lysate, rat skin tissue lysate.
Subcellular location: Nucleus, cytoplasm; Perinuclear region.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q99704 Human | Q4QQV2 Rat
Alternative names: Docking protein 1 62kD Docking protein 1 DOK 1 DOK1 DOK1_HUMAN Downstream of tyrosine kinase 1 p62(dok) P62DOK pp62
Images
ET7110-75_1.jpg Fig1: Western blot analysis of DOK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Rat skin tissue lysate
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.