DOK1 Recombinant Rabbit Monoclonal Antibody [JE54-52]
cat.: ET7110-75
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE54-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19/37/52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DOK1 aa 360-481 / 481. |
| Positive control: | K562 cell lysate, rat skin tissue lysate, mouse pancreas tissue lysates. |
| Subcellular location: | Nucleus, cytoplasm; Perinuclear region. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q99704 Human | Q4QQV2 Rat | P97465 Mouse |
| Alternative names: | Docking protein 1 62kD Docking protein 1 DOK 1 DOK1 DOK1_HUMAN Downstream of tyrosine kinase 1 p62(dok) P62DOK pp62 |
Images
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Fig1:
Western blot analysis of DOK1 on different lysates with Rabbit anti-DOK1 antibody (ET7110-75) at 1/1,000 dilution. Lane 1: K562 cell lysate Lane 2: Rat skin tissue lysate(20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 19/37/52 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-75) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of DOK1 on mouse pancreas tissue lysates with Rabbit anti-DOK1 antibody (ET7110-75) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 19/37/52 kDa Observed band size: 35 kDa Exposure time: 2 minutes; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-75) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.