GAA Recombinant Rabbit Monoclonal Antibody [JE54-59]
cat.: ET7110-77
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-59 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105/76/70 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GAA aa 120-230 / 952. |
| Positive control: | HepG2 cell lysate, MCF-7 cell lysate, human placenta tissue lysate, human liver tissue, human placenta tissue, human liver tissue, human liver carcinoma tissue, HepG2. |
| Subcellular location: | Lysosome, lysosome membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:1,000 |
| Uniprot #: | SwissProt: P10253 Human |
| Alternative names: | 70 kDa lysosomal alpha-glucosidase Acid alpha glucosidase Acid maltase Aglucosidase alfa Alpha glucosidase GAA Glucosidase alpha acid (Pompe disease glycogen storage disease type II) Glucosidase alpha acid Glucosidase alpha LYAG LYAG_HUMAN Lysosomal alpha glucosidase |
Images
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Fig1:
Western blot analysis of GAA on different lysates with Rabbit anti-GAA antibody (ET7110-77) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 105 kDa Observed band size: 110,76,95 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-77) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of GAA on different lysates with Rabbit anti-GAA antibody (ET7110-77) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si GAA cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 105 kDa Observed band size: 75-110 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-GAA antibody (ET7110-77) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-77) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GAA antibody (ET7110-77) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GAA antibody (ET7110-77) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of GAA was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-77, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.