GAA Recombinant Rabbit Monoclonal Antibody [JE54-59]
cat.: ET7110-77
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE54-59 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105/76/70 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GAA aa 120-230 / 952. |
| Positive control: | HepG2 cell lysate, MCF-7 cell lysate, human placenta tissue lysate, human liver tissue, human placenta tissue, human liver tissue, human liver carcinoma tissue, HepG2. |
| Subcellular location: | Lysosome, lysosome membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P FC |
1:500-1:2,000 1:50-1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P10253 Human |
| Alternative names: | 70 kDa lysosomal alpha-glucosidase Acid alpha glucosidase Acid maltase Aglucosidase alfa Alpha glucosidase GAA Glucosidase alpha acid (Pompe disease glycogen storage disease type II) Glucosidase alpha acid Glucosidase alpha LYAG LYAG_HUMAN Lysosomal alpha glucosidase |
Images
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Fig1:
Western blot analysis of GAA on different lysates with Rabbit anti-GAA antibody (ET7110-77) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF-7 cell lysate Lane 3: Human placenta tissue lysate(20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 105/76/70 kDa Observed band size: 99/76 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-77) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling GAA with Rabbit anti-GAA antibody (ET7110-77) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7110-77, red) at 1/50 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling GAA with Rabbit anti-GAA antibody (ET7110-77) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7110-77, red) at 1/50 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GAA antibody (ET7110-77) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-77) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-GAA antibody (ET7110-77) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-77) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GAA antibody (ET7110-77) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-77) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Flow cytometric analysis of GAA was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-77, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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