APLP2 Recombinant Rabbit Monoclonal Antibody [JE54-62]
cat.: ET7110-78
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human APLP2 aa 201-300 / 763. |
| Positive control: | Rat brian tissue lysate, human placenta tissue lysate, Hela cell lysate, rat testis tissue, human kidney tissue. |
| Subcellular location: | Nucleus, cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q06481 Human | P15943 Rat |
| Alternative names: | amyloid beta (A4) precursor like protein 2 Amyloid like protein 2 Amyloid precursor protein homolog HSD 2 amyloid precursor protein homolog HSD2 Amyloid protein homolog Amyloid-like protein 2 APLP-2 Aplp2 APLP2_HUMAN APPH APPL2 CDEBP CDEI box binding protein CDEI box-binding protein |
Images
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Fig1:
Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (ET7110-78) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD APLP2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 2 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-78) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (ET7110-78) at 1/500 dilution. Lane 1: Rat brian tissue lysate(20 µg/Lane) Lane 2: Human placenta tissue lysate(20 µg/Lane) Lane 3: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 100/120 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-78) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-APLP2 antibody (ET7110-78) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-APLP2 antibody (ET7110-78) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.