Alpha SNAP Recombinant Rabbit Monoclonal Antibody [JE54-66]
cat.: ET7110-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE54-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Alpha SNAP aa 161-295 / 295. |
| Positive control: | Human placenta tissue lysate, mouse testis tissue lysate, SH-SY5Y cell lysate, SiHa. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P54920 Human | Q9DB05 Mouse |
| Alternative names: | Alpha soluble NSF attachment protein Alpha-soluble NSF attachment protein N ethylmaleimide sensitive factor attachment protein alpha N-ethylmaleimide-sensitive factor attachment protein alpha napA NSF attachment protein alpha SNAA_HUMAN SNAP alpha SNAP-alpha SNAPA |
Images
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Fig1:
Western blot analysis of Alpha SNAP on different lysates with Rabbit anti-Alpha SNAP antibody (ET7110-79) at 1/500 dilution. Lane 1: Human placenta tissue lysate(20 µg/Lane) Lane 2: Mouse testis tissue lysate 20 µg/Lane. Lane 3: SH-SY5Y cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-79) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of SiHa cells labeling Alpha SNAP with Rabbit anti-Alpha SNAP antibody (ET7110-79) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alpha SNAP antibody (ET7110-79) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.