DPYD Recombinant Rabbit Monoclonal Antibody [JE54-79]
cat.: ET7110-83
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 111 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DPYD aa 831-970 / 1,025. |
| Positive control: | THP-1 cell lysates, SiHa cell lysates, human liver tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q12882 Human |
| Alternative names: | DHP DHPDHase Dihydropyrimidine dehydrogenase [NADP(+)] Dihydropyrimidine dehydrogenase [NADP+] Dihydropyrimidine dehydrogenase Dihydrothymine dehydrogenase Dihydrouracil dehydrogenase DPD DPYD DPYD_HUMAN MGC132008 MGC70799 OTTHUMP00000058954 |
Images
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Fig1:
Western blot analysis of DPYD on THP-1 cell lysates with Rabbit anti-DPYD antibody (ET7110-83) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 111 kDa Observed band size: 111 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-83) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of DPYD on SiHa cell lysates with Rabbit anti-DPYD antibody (ET7110-83) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 111 kDa Observed band size: 111 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-83) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DPYD antibody (ET7110-83) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-83) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.