LGI1 Recombinant Rabbit Monoclonal Antibody [JE54-88]
cat.: ET7110-86
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE54-88 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 64 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LGI1 aa 130-270 / 557. |
| Positive control: | A431 cell lysate, A172 cell lysate, MG-63 cell lysate, mouse hippocampus tissue lysate. |
| Subcellular location: | Secreted, synapse. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: O95970 Human | Q9JIA1 Mouse |
| Alternative names: | ADLTE ADPAEF ADPEAF Epitempin 1 EPITEMPIN Epitempin-1 EPT ETL1 IB1099 leucine rich glioma inactivated 1 Leucine rich glioma-inactivated protein 1 Leucine-rich glioma-inactivated protein 1 LGI1 LGI1_HUMAN OTTHUMP00000020121 OTTHUMP00000020122 |
Images
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Fig1:
Western blot analysis of LGI1 on different lysates with Rabbit anti-LGI1 antibody (ET7110-86) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: A172 cell lysate Lane 3: MG-63 cell lysate Lane 4: Mouse hippocampus tissue lysate(20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-86) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.