PEX19 Recombinant Rabbit Monoclonal Antibody [JE54-93]
cat.: ET7110-89
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PEX19 aa 1-140 / 299. |
| Positive control: | Raji cell lysate, Jurkat cell lysate, HeLa cell lysate, C6 cell lysate, Rat liver tissue lysate, rat liver tissue, rat small intestine tissue. |
| Subcellular location: | Peroxisome membrane, cytoplasmic side, cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P40855 Human | Q9QYU1 Rat |
| Alternative names: | 33 kDa housekeeping protein D1S2223E HK33 Housekeeping gene 33kD OK/SW-cl.22 PBD12A Peroxin 19 Peroxin-19 Peroxisomal biogenesis factor 19 Peroxisomal farnesylated protein PEX19 PEX19_HUMAN PMP1 PMPI PXF PXMP1 |
Images
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Fig1:
Western blot analysis of PEX19 on different lysates with Rabbit anti-PEX19 antibody (ET7110-89) at 1/5,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 33 kDa Observed band size: 40 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-89) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PEX19 antibody (ET7110-89) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-89) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-PEX19 antibody (ET7110-89) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-89) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.