Kallikrein 5 Recombinant Rabbit Monoclonal Antibody [JE54-94]
cat.: ET7110-90
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE54-94 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Kallikrein 5 aa 161-293 / 293. |
| Positive control: | MCF-7 cell lysate, SK-Br-3 cell lysate, MCF-7, A431. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y337 Human |
| Alternative names: | HK5 Kallikrein 5 Kallikrein L2 Kallikrein like 2 Kallikrein like protein 2 Kallikrein related peptidase 5 Kallikrein-5 Kallikrein-like protein 2 Kallikrein5 KLK 5 KLK L2 KLK-L2 KLK5 KLK5_HUMAN KLKL 2 KLKL2 SCTE Stratum corneum tryptic enzyme UNQ570/PRO1132 |
Images
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Fig1:
Western blot analysis of Kallikrein 5 on different lysates with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: SK-Br-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 32 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3: Flow cytometric analysis of Kallikrein 5 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.