Arg2 Recombinant Rabbit Monoclonal Antibody [JE54-97]
cat.: ET7110-91
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-97 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Arg2 aa 305-354 / 354. |
| Positive control: | A549 cell lysate, Human small intestine tissue lysate, Human kidney tissue lysate, K562 cell lysate, human liver tissue, human prostate carcinoma tissue, human kidney tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P78540 Human |
| Alternative names: | ARG2 ARGI2_HUMAN Arginase II mitochondrial Arginase type II Arginase-2 arginase-2, mitochondrial Kidney arginase Kidney type arginase Kidney-type arginase L arginine amidinohydrolase L arginine ureahydrolase mitochondrial Non hepatic arginase Non-hepatic arginase Nonhepatic arginase Type II arginase |
Images
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Fig1:
Western blot analysis of Arg2 on different lysates with Rabbit anti-Arg2 antibody (ET7110-91) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Arg2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-91) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Arg2 on different lysates with Rabbit anti-Arg2 antibody (ET7110-91) at 1/1,000 dilution. Lane 1: HepG2 Lane 2: K-562 Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7110-91, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 39 kDa Observed band size: 39 kDa |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Arg2 antibody (ET7110-91) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-91) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Arg2 antibody (ET7110-91) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-91) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Arg2 antibody (ET7110-91) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-91) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.