Arg2 Recombinant Rabbit Monoclonal Antibody [JE54-97]
cat.: ET7110-91
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE54-97
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Arg2 aa 305-354 / 354.
Positive control: Human small intestine tissue lysate, human kidney tissue lysate, K562 cell lysate, RWPE-1, SW620, human liver tissue, human prostate carcinoma tissue, human kidney tissue, 293.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P78540 Human
Alternative names: ARG2 ARGI2_HUMAN Arginase II mitochondrial Arginase type II Arginase-2 arginase-2, mitochondrial Kidney arginase Kidney type arginase Kidney-type arginase L arginine amidinohydrolase L arginine ureahydrolase mitochondrial Non hepatic arginase Non-hepatic arginase Nonhepatic arginase Type II arginase
Images
ET7110-91_1.jpg Fig1: Western blot analysis of Arg2 on different lysates with Rabbit anti-Arg2 antibody (ET7110-91) at 1/1,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD Arg2 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 40 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-91) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7110-91_2.jpg Fig2: Western blot analysis of Arg2 on different lysates with Rabbit anti-Arg2 antibody (ET7110-91) at 1/500 dilution.

Lane 1: Human small intestine tissue lysate
Lane 2: Human kidney tissue lysate
Lane 3: K562 cell lysate(10 µg/Lane)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-91) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7110-91_3.jpg Fig3: Immunocytochemistry analysis of RWPE-1 cells labeling Arg2 with Rabbit anti-Arg2 antibody (ET7110-91) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Arg2 antibody (ET7110-91) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7110-91_4.jpg Fig4: Immunocytochemistry analysis of SW620 cells labeling Arg2 with Rabbit anti-Arg2 antibody (ET7110-91) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Arg2 antibody (ET7110-91) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7110-91_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Arg2 antibody (ET7110-91) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-91) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-91_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Arg2 antibody (ET7110-91) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-91) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-91_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Arg2 antibody (ET7110-91) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-91) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-91_8.jpg Fig8: Flow cytometric analysis of Arg2 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-91, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.