RanBP9 Recombinant Rabbit Monoclonal Antibody [JE55-10]
cat.: ET7110-95
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 116 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RANBP9 aa 90-205 / 1,041. |
| Positive control: | Mouse hippocampus tissue lysate, rat kidney tissue lysate, SK-Br-3 cell lysate, rat testis tissue, human kidney tissue, mouse heart tissue. |
| Subcellular location: | Nucleus, cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q96P70 Human | Q91YE6 Mouse Entrez Gene: 304817 Rat |
| Alternative names: | Imp9 Imp9a Imp9b Importin-9 Importin-9a Ipo9 IPO9_HUMAN Ran-binding protein 9 RanBP9 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of RanBP9 on different lysates with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/1,000 dilution. Lane 1: C2C12 (Mouse myoblasts) cell lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat testis tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7110-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 116.0 kDa Observed band size: 110 kDa |
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Fig5:
Western blot analysis of RanBP9 on different lysates with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/1,000 dilution. Lane 1: Jurkat (Human acute T-cell leukemia cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7110-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 116.0 kDa Observed band size: 110 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.