RanBP9 Recombinant Rabbit Monoclonal Antibody [JE55-10]
cat.: ET7110-95
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 116 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RANBP9 aa 90-205 / 1,041. |
| Positive control: | Mouse hippocampus tissue lysate, rat kidney tissue lysate, SK-Br-3 cell lysate, rat testis tissue, human kidney tissue, mouse heart tissue. |
| Subcellular location: | Nucleus, cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q96P70 Human | Q91YE6 Mouse Entrez Gene: 304817 Rat |
| Alternative names: | Imp9 Imp9a Imp9b Importin-9 Importin-9a Ipo9 IPO9_HUMAN Ran-binding protein 9 RanBP9 |
Images
|
Fig1:
Western blot analysis of RanBP9 on different lysates with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/500 dilution. Lane 1: Mouse hippocampus tissue lysate (20 µg/Lane) Lane 2: Rat kidney tissue lysate (20 µg/Lane) Lane 3: SK-Br-3 cell lysate (10 µg/Lane) Predicted band size: 116 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-95) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.