Glycerol Kinase Recombinant Rabbit Monoclonal Antibody [JE55-12]
cat.: ET7110-96
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE55-12
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size 61/57 kDa.
Isotype: IgG
Immunogen: Recombinant protein within Human Glycerol kinase aa 440-559 / 559.
Positive control: Mouse liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, mouse bone marrow tissue lysate, rat testis tissue lysate, rat testis tissue, human liver tissue, human kidney tissue, mouse testis tissue, 293.
Subcellular location: Mitochondrion outer membrane, cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P32189 Human | Q64516 Mouse | Q63060 Rat
Alternative names: ATP glycerol 3 phosphotransferase ATP:glycerol 3 phosphotransferase ATP:glycerol 3-phosphotransferase D930012N15Rik GK GK1 GKD GLPK_HUMAN Glycerokinase Glycerol kinase OTTHUMP00000023108 OTTHUMP00000023109 OTTHUMP00000215321
Images
ET7110-96_1.jpg Fig1: Western blot analysis of Glycerol Kinase on different lysates with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/1,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si Glycerol Kinase cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 61/57 kDa
Observed band size: 61/57 kDa

Exposure time: 1 minute; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-96) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7110-96_2.jpg Fig2: Western blot analysis of Glycerol Kinase on different lysates with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/500 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat kidney tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 61/57 kDa
Observed band size: 61/57 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-96) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7110-96_3.jpg Fig3: Western blot analysis of Glycerol Kinase on different lysates with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/500 dilution.

Lane 1: Mouse bone marrow tissue lysate
Lane 2: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 61/57 kDa
Observed band size: 61 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-96) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7110-96_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer(pH 8.0-8.4))for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-96) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-96_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer(pH 8.0-8.4))for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-96) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-96_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer(pH 8.0-8.4))for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-96) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-96_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Glycerol Kinase antibody (ET7110-96) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer(pH 8.0-8.4))for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-96) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7110-96_8.jpg Fig8: Flow cytometric analysis of Glycerol Kinase was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-96, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.