LMAN1 Recombinant Rabbit Monoclonal Antibody [JE55-19]
cat.: ET7110-98
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LMAN1 aa 281-405 / 510. |
| Positive control: | NIH/3T3 cell lysate, human placenta tissue lysate, HUVEC cell lysate, rat brain tissue, mouse kidney tissue, mouse testis tissue. |
| Subcellular location: | Golgi apparatus membrane, endoplasmic reticulum membrane, endoplasmic reticulum-golgi intermediate compartment membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P49257 Human | Q9D0F3 Mouse | Q62902 Rat |
| Alternative names: | Endoplasmic reticulum golgi intermediate compartment protein 53 ER-Golgi intermediate compartment 53 kDa protein ERGIC-53 ERGIC53 ERGIC53 like protein F5F8D FMFD1 Gp58 Intracellular mannose specific lectin Intracellular mannose-specific lectin MR60 Lectin mannose binding 1 Lectin mannose-binding 1 Lman1 LMAN1 like protein LMAN1_HUMAN MCFD1 MR60 Protein ERGIC-53 |
Images
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Fig1:
Western blot analysis of LMAN1 on different lysates with Rabbit anti-LMAN1 antibody (ET7110-98) at 1/500 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: Human placenta tissue lysate(20 µg/Lane) Lane 3: HUVEC cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-98) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LMAN1 antibody (ET7110-98) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-98) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-LMAN1 antibody (ET7110-98) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-98) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-LMAN1 antibody (ET7110-98) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-98) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.