PDLIM1 Recombinant Rabbit Monoclonal Antibody [JE55-21]
cat.: ET7110-99
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE55-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PDLIM1 aa 181-285 / 329. |
| Positive control: | Human placenta tissue lysate, mouse lung tissue lysate, HepG2 cell lysate, rat heart tissue, HepG2, human placenta tissue, A431 cell lysate. |
| Subcellular location: | Cytoskeleton, cytoplasm, Z line. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:2,000 1:50-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: O00151 Human | O70400 Mouse | Q3TZ17 Mouse | P52944 Rat |
| Alternative names: | C terminal LIM domain protein 1 C-terminal LIM domain protein 1 Carboxy terminal LIM domain protein 1 CLIM 1 CLIM1 CLP 36 CLP36 Elfin Enigma Enigma homolog hCLIM 1 hCLIM1 LIM domain protein CLP 36 LIM domain protein CLP-36 LIM domain protein CLP36 PDLI1_HUMAN PDLIM 1 Pdlim1 PDZ and LIM domain 1 PDZ and LIM domain protein 1 |
Images
|
Fig1:
Western blot analysis of PDLIM1 on different lysates with Rabbit anti-PDLIM1 antibody (ET7110-99) at 1/500 dilution. Lane 1: Human placenta tissue lysate Lane 2: Mouse lung tissue lysate Lane 3: HepG2 cell lysate(10 µg/Lane) Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-99) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-PDLIM1 antibody (ET7110-99) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-99) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Flow cytometric analysis of PDLIM1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-99, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-PDLIM1 antibody (ET7110-99) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of PDLIM1 on different lysates with Rabbit anti-PDLIM1 antibody (ET7110-99) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-99) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.