LPP Recombinant Rabbit Monoclonal Antibody [JE55-29]
cat.: ET7111-02
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE55-29 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LPP aa 1-140 / 612. |
| Positive control: | Daudi cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Mouse small intestine tissue lysate, Rat skin tissue lysate, Mouse smooth muscle tissue lysate, rat bladder tissue, human kidney intestine tissue, human small intestine tissue. |
| Subcellular location: | Nucleus, cell membrane, cytoplasm, cell junction. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q93052 Human | Q8BFW7 Mouse | Q5XI07 Rat |
| Alternative names: | DKFZp779O0231 FLJ30652 FLJ41512 LIM domain containing preferred translocation partner in lipoma LIM domain-containing preferred translocation partner in lipoma LIM protein Lipoma preferred partner Lipoma-preferred partner lpp LPP_HUMAN |
Images
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Fig1:
Western blot analysis of LPP on different lysates with Rabbit anti-LPP antibody (ET7111-02) at 1/1,000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse small intestine tissue lysate (40 µg/Lane) Predicted band size: 66 kDa Observed band size: 70 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-02) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of LPP on different lysates with Rabbit anti-LPP antibody (ET7111-02) at 1/500 dilution. Lane 1: Rat skin tissue lysate Lane 2: Mouse smooth muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-02) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-LPP antibody (ET7111-02) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-02) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney intestine tissue with Rabbit anti-LPP antibody (ET7111-02) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-02) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-LPP antibody (ET7111-02) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-02) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.