TEAD1 Recombinant Rabbit Monoclonal Antibody [JE52-71]
cat.: ET7111-04
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE52-71
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human TEAD1 aa 1-50 / 426.
Positive control: A431 cell lysate, A549 cell lysate, HeLa cell lysate, Mouse embryo tissue lysate, Mouse skeletal muscle tissue lysate, Rat embryo tissue lysate, Rat skeletal muscle tissue lysate, human colon cancer tissue, human testis tissue, A549, MG-63, SiHa.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: P28347 Human | P30051 Mouse
Entrez Gene: 361630 Rat
Alternative names: AA Atrophia areata peripapillary chorioretinal degeneration NTEF 1 NTEF-1 NTEF1 Protein GT IIC Protein GT-IIC REF 1 REF1 SV40 transcriptional enhancer factor TCF 13 TCF-13 TCF13 TEA domain family member TEA domain family member 1 (SV40 transcriptional enhancer factor) TEA domain family member 1 TEAD 1 TEAD 1 protein TEAD-1 TEAD1 TEAD1 protein TEAD1_HUMAN TEF 1 TEF1 Transcription factor 13 (SV40 transcriptional enhancer factor) Transcription factor 13 Transcriptional enhancer factor 1 Transcriptional enhancer factor TEF-1 Transcriptional enhancer factor TEF1
Images
ET7111-04_1.jpg Fig1: All lanes: Western blot analysis of TEF1 with anti-TEF1 antibody [JE52-71] (ET7111-04) at 1:500 dilution.

Lane 1: Wild-type ct26.wt whole cell lysate.
Lane 2: TEF1 knockout ct26.wt whole cell lysate.

ET7111-04 was shown to specifically react with TEF1 in wild-type ct26.wt cells. No band was observed when TEF1 knockout samples were tested. Wild-type and TEF1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-TEF1 antibody (ET7111-04, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
ET7111-04_2.jpg Fig2: Western blot analysis of TEAD1 on different lysates with Rabbit anti-TEAD1 antibody (ET7111-04) at 1/1,000 dilution.

Lane 1: A431 cell lysate
Lane 2: A549 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Mouse embryo tissue lysate
Lane 5: Mouse skeletal muscle tissue lysate
Lane 6: Rat embryo tissue lysate
Lane 7: Rat skeletal muscle tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 48 kDa
Observed band size: 48/50 kDa

Exposure time: 2 minutes 7 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7111-04_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-TEAD1 antibody (ET7111-04) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-04_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TEAD1 antibody (ET7111-04) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7111-04_5.jpg Fig5: Immunocytochemistry analysis of A549 cells labeling TEAD1 with Rabbit anti-TEAD1 antibody (ET7111-04) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TEAD1 antibody (ET7111-04) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7111-04_6.jpg Fig6: Immunocytochemistry analysis of MG-63 cells labeling TEAD1 with Rabbit anti-TEAD1 antibody (ET7111-04) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TEAD1 antibody (ET7111-04) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7111-04_7.jpg Fig7: Immunocytochemistry analysis of SiHa cells labeling TEAD1 with Rabbit anti-TEAD1 antibody (ET7111-04) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TEAD1 antibody (ET7111-04) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.