TEF1 Recombinant Rabbit Monoclonal Antibody [JE52-71]
cat.: ET7111-04
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE52-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TEF1 aa 1-50 / 426. |
| Positive control: | SiHa cell lysate, Hela cell lysate, human colon cancer tissue using anti-商品名 antibody. The section was pre-treated using heat mediated antigen retrieval, rat skeletal muscle tissue using anti-商品名 antibody. The section was pre-treated using heat mediated antigen retrieval. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: P28347 Human | P30051 Mouse |
| Alternative names: | AA Atrophia areata peripapillary chorioretinal degeneration NTEF 1 NTEF-1 NTEF1 Protein GT IIC Protein GT-IIC REF 1 REF1 SV40 transcriptional enhancer factor TCF 13 TCF-13 TCF13 TEA domain family member TEA domain family member 1 (SV40 transcriptional enhancer factor) TEA domain family member 1 TEAD 1 TEAD 1 protein TEAD-1 TEAD1 TEAD1 protein TEAD1_HUMAN TEF 1 TEF1 Transcription factor 13 (SV40 transcriptional enhancer factor) Transcription factor 13 Transcriptional enhancer factor 1 Transcriptional enhancer factor TEF-1 Transcriptional enhancer factor TEF1 |
Images
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Fig1:
All lanes: Western blot analysis of TEF1 with anti-TEF1 antibody [JE52-71] (ET7111-04) at 1:500 dilution. Lane 1: Wild-type ct26.wt whole cell lysate. Lane 2: TEF1 knockout ct26.wt whole cell lysate. ET7111-04 was shown to specifically react with TEF1 in wild-type ct26.wt cells. No band was observed when TEF1 knockout samples were tested. Wild-type and TEF1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-TEF1 antibody (ET7111-04, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2:
Western blot analysis of TEF1 on different lysates with Rabbit anti-TEF1 antibody (ET7111-04) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-04) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of A549 cells labeling TEF1 with Rabbit anti-TEF1 antibody (ET7111-04) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TEF1 antibody (ET7111-04) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of MG-63 cells labeling TEF1 with Rabbit anti-TEF1 antibody (ET7111-04) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TEF1 antibody (ET7111-04) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of SiHa cells labeling TEF1 with Rabbit anti-TEF1 antibody (ET7111-04) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TEF1 antibody (ET7111-04) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-TEF1 antibody (ET7111-04) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-TEF1 antibody (ET7111-04) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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