CD35 Recombinant Rabbit Monoclonal Antibody [JE54-31]
cat.: ET7111-05
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 224 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD35 aa 280-372 / 2,039. |
| Positive control: | Human tonsil tissue, human spleen tissue, human kidney tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IHC-P WB |
1:50-1:200 1:500 |
| Uniprot #: | SwissProt: P17927 Human |
| Alternative names: | C3 binding protein C3b/C4b receptor C3BR C4BR CD 35 CD35 CD35 antigen complement component (3b/4b) receptor 1 (Knops blood group) complement component (3b/4b) receptor 1 including Knops blood group system Complement component receptor 1 Complement receptor 1 Complement receptor type 1 CR 1 CR1 CR1_HUMAN KN Knops blood group antigen |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD35 antibody (ET7111-05) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-05) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD35 antibody (ET7111-05) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-05) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD35 antibody (ET7111-05) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-05) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.