Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P |
Clonality: | Monoclonal |
Clone number: | JE53-81 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size 87 kDa. |
Isotype: | IgG |
Immunogen: | Synthetic peptide within N terminal Human PLAP. |
Positive control: | Human prostate carcinoma tissue, human uterus tissue. |
Subcellular location: | Nucleus, cytoplasm, synapse. |
Recommended Dilutions:
IHC-P |
1:50-1:200 |
Uniprot #: | SwissProt: Q9Y263 Human |
Alternative names: | FLJ11281 FLJ12699 Glycerophosphatase OTTHUMP00000045176 Phospholipase A 2 activating protein Phospholipase A-2-activating protein Phospholipase A2 activating protein PLA2P Plaa PLAP PLAP_HUMAN |
Fig1:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-PLAP antibody (ET7111-06) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-06) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-PLAP antibody (ET7111-06) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-06) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |